Colorimetric substrate for dehydrogenases and other oxidases. NBT is used with X-Phos for colorimetric detection of alkaline phosphatase activity in blotting, immunohistochemical and cytochemistry techniques. Protect from light.
Common Name: [Nitro Blue Tetrazolium] [2,2′-Di-P-Nitrophenyl-5, 5′-Diphenyl-3, 3′-(3,3′-Dimethoxy-4, 4′-Diphenylene)-Ditetrazolium Chloride]
CAS Number: 298-83-9
Molecular Weight: 817.6
Chemical Formula: C40H30Cl2N10O6
Solubility: 1% Soluble in hot methanol
Storage Temperature: 2-8C
Storage Conditions: Refrigerator
Appearance: Yellow Crystalline Powder
Identification: Conforms
IR spectrum: Concordant
Loss on Drying: 9.5 %
Molar Absorptivity in Methyl Alcohol @257 nm: 63000
Purity: 98 %
Solubility: Clear, Yellow Solution
Solubility (0.5% in H2O): Clear yellow solution
Solubility 1% in hot methanol: Clear yellow solution
Sulfated Ash: 0.2 %
Shipping Weight: 0.06 lbs
Shipping Dimensions: 1.25 x 1.75 x 1.25
Research or further manufacturing use only, not for food or drug use.